Anti-Active Rab35 Mouse Monoclonal Antibody_一抗_免疫学_科研试剂_供应

价格:4800.00/支
品牌:NewEastBio
货号:26922
包装:30 µL
起订:1支
供应:30000支
发货:1天内

Active Rab35-GTP [26922]
[30 µL]

Catalog Number: 26922

Gene Symbol: RAB35

Description: Anti-Active Rab35 Mouse Monoclonal Antibody

Background: The Rab family of GTPases regulates eukaryotic vesicular membrane traffic. Rab35 is with both plasma membrane and endosomal localization, and has been implicated in diverse processes that include T-cell receptor recycling, oocyte yolk protein recycling and cytokinesis. Rab35 regulates neurite outgrowth in neuronal-like cells, and can induce protrusions.

Immunogen: Recombinant full length protein of active Rab35

Applications: IP, IHC, IF

Recommended Dilutions:
1 µg for ~2 mg total cellular proteins for IP
1:100 dilution for IHC, IF

Concentration: 1 mg/ml

Host: mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgG

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Anti-active Rab35 antibody recognizes active Rab35 proteins of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Immunoprecipitation/Western blot:

Rab35 activation assay. Purified full-length Rab35 proteins were immunoprecipitated after treated with GDP (lane 1) orGTPγS (lane 2). Immunoprecipitation was done with the anti-active Rab35 monoclonal antibody (Cat. No. 26922). Immunoblot was with an anti-Rab35 polyclonal antibody (Cat. No. 21078).