Anti-IDH2 (R140Q) Mouse Monoclonal Antibody_一抗_免疫学_科研试剂_供应

价格:4800.00/支
品牌:NewEastBio
货号:26165
包装:100 µL
起订:1支
供应:30000支
发货:1天内

IDH2 (R140Q) [26165]
[100ul]

Catalog Number: 26165

Gene Symbol: IDH2; D2HGA2; ICD-M; IDH; IDHM; IDP; IDPM; mNADP-IDH

Description: Anti-IDH2 (R140Q) Mouse Monoclonal Antibody

Background: Isocitrate dehydrogenase (IDH) catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations of Arg-132 in IDH to Cys, His, Leu or Ser abolish magnesium binding and the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors.

Immunogen: A synthetic peptide from the internal region of IDH2 which includes the mutation of R140Q, human origin.

Applications: ELISA, WB, IF, IHC

Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:500-1:1000
IF: 1:50-1:100
IHC: 1:50-1:100

Concentration: 1 mg/ml

Host: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype:

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes R140Q mutant, but not wild-type IDH2 of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Western blot:

Western blot analysis of recombinant IDH2 (R140Q) and wildtype proteins. Purified His-tagged IDH2 (R140Q) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with anti-IDH2 (R140Q) monoclonal antibody (Cat. #26165).

Immunofluorescence:

Immunofluorescence of cells expressing IDH2 proteins with anti-IDH2 (R140Q) antibody. HEK293T cells were transfected with pCDNA3-GFP-IDH2 (WT) plasmid (left column) or pCDNA3-GFP-IDH2 (R140Q) plasmid (right column), then fixed and stained with anti-IDH2 (R140Q) monoclonal antibody (Cat. #26165).