Anti-IDH2 (R172H) Mouse Monoclonal Antibody_一抗_免疫学_科研试剂_供应

价格:4800.00/支
品牌:NewEastBio
货号:26084
包装:100 µL
起订:1支
供应:30000支
发货:1天内

IDH2 (R172H) [26084]
[100 µL]

Catalog Number: 26084

Gene Symbol: IDH2

Description: Anti-IDH2 (R172H) Mouse Monoclonal Antibody

Background: Isocitrate dehydrogenase (IDH) catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations of Arg-172 in IDH2 to Cys, His, Leu or Ser abolish magnesium binding and the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors.

Immunogen: A synthetic peptide from the internal region of human IDH2 which includes the mutation of R172H.

Applications: ELISA, WB, IHC, IF

Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:100-1:1000
IHC: 1:50-1:100
IF: 1:50-1:100

Concentration: 1 mg/ml

Host: mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgG

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Anti-IDH2(R172H) antibody recognizes IDH2(R172H) of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles.

Western blot:

	Western blot analysis of recombinant IDH2(R172H) and wild-type IDH2 proteins. Purified his-tagged IDH2(R172H) protein (Cat. # 10201) and wild-type IDH2 protein (Cat. # 10200) were blotted with anti-IDH2(R172H) monoclonal antibody (Cat. # 26084) (left panel). Proteins loading were blotted with anti-IDH2 monoclonal antibody (right panel).
Immunofluorescence:

Immunofluorescence of cells expressing IDH2 proteins with anti-IDH2 (R172H) antibody. HEK293T cells were transfected with pCDNA3-GFP-IDH2 (WT) plasmid (left column) or pCDNA3-GFP-IDH2 (R172H) plasmid (right column), then fixed and stained with anti-IDH2 (R172H) monoclonal antibody (Cat. #26084).